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Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)

The most common health problem in Indonesia is bacterial infection. Dysphania ambrosioides L., also known as epazote leaves, is an herbal plant that has antimicrobial potential. This research was conducted to see at what concentration there is antibacterial activity of epazote leaf infusion Dysphania ambrosioides L. against Escherichia coli bacteria. This laboratory experimental study used a complete randomized design (RAL) research. Antibacterial activity testing of epazote leaves was carried out using the disc paper diffusion method with concentrations of 50%, 75%, and 100%. Epazote leaves are boiled in an infusion pan for 15 minutes, until the temperature reaches 90 degrees Celsius. Ampicillin positive control 10 μg/disc. Based on the test results of secondary metabolites of positive epazote leaf infusion there are alkaloids, flavonoids, tannins, saponins, triterpenoids, and phenolics. The results of the study showed that infusion of epazote leaves inhibited Escherichia coli bacteria by diameter inhibition. Average at a concentration of 50% = 14.04 mm, 75% = 16.92 mm, 100% = 17.94 mm, which means that there is antibacterial activity of epazote leaf infusion against Escherichia coli bacteria.

The results showed that the infusion of epazote leaves inhibited Escherichia coli bacteria.However, further research is needed to make epazote leaves safer for public consumption.Based on statistical tests, epazote leaf infusion at a 50% concentration has the same effect as 75% and 100% concentrations, indicating that using a 50% concentration is sufficient to inhibit the growth of Escherichia coli bacteria.

Penelitian lebih lanjut perlu dilakukan untuk mengidentifikasi senyawa spesifik dalam infusa daun epazote yang bertanggung jawab atas aktivitas antibakterinya, sehingga dapat dimanfaatkan secara optimal dalam pengembangan obat herbal. Selain itu, studi toksisitas yang komprehensif perlu dilakukan untuk memastikan keamanan penggunaan infusa daun epazote sebagai alternatif pengobatan infeksi bakteri, terutama dalam jangka panjang. Sebagai pengembangan, penelitian dapat difokuskan pada formulasi infusa daun epazote menjadi sediaan farmasi yang lebih praktis dan stabil, seperti kapsul atau salep, untuk meningkatkan efektivitas dan kemudahan penggunaannya. Penelitian lanjutan juga dapat mengeksplorasi potensi sinergis antara infusa daun epazote dengan antibiotik konvensional, untuk mengatasi masalah resistensi bakteri dan meningkatkan efektivitas terapi. Terakhir, studi epidemiologi dapat dilakukan untuk mengkaji efektivitas penggunaan tradisional daun epazote dalam mengatasi infeksi bakteri di masyarakat, serta mengidentifikasi potensi manfaat dan risiko yang terkait.

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