UBUB

The Journal of Experimental Life ScienceThe Journal of Experimental Life Science

Rheumatoid arthritis (RA) is an autoimmune disease that can cause progressive damage to the joints of patients. The number of patients is expected to increase, along with the exact cause of this disease remains unknown. However, there are several risk factors associated with RA, including dysbiosis. The purpose of this study was to characterize the composition of intestinal microbiota in the RA and control groups through fecal analysis and reveal the association of microbiota composition with RA disease in Indonesia, especially Malang. Fecal samples were obtained from RA patients and controls. Fecal analysis was carried out through several stages, namely the calculation of total bacterial colonies, isolation and characterization of anaerobic bacteria, calculation of the Simpson diversity index, and DNA isolation. Analysis of bacterial composition profiles in fecal was carried out using 6 specific primer sets through PCR analysis. The results of the 16S rRNA PCR analysis showed different microbiota compositions between RA patients and controls. The number of Enterococcus bacterial group was lower in the control patients than the RA group, whereas the Lactobacillus bacteria decreased in RA patients. In addition, our study found that the existence of bacterial isolate 11 changed the composition of microbiota in RA patients, and the DNA band only appeared in Universal primers. The diversity of bacterial species can provide symbiotic and pathogenetic effects in RA patients.

This study found that Enterococcus was the bacterial group found in both the control and RA patients in Malang.The abundance of Enterococcus in RA patients was higher than in the control patients.The bacterial group in control patients was dominated by the Lactobacillus bacteria, which was not found in the RA patients.Further analysis is required to determine the bacterial group of isolate 11 in the RA patients.

Penelitian lebih lanjut perlu dilakukan untuk mengidentifikasi secara spesifik bakteri isolate 11 yang hanya ditemukan pada pasien RA, karena keberadaannya dapat menjadi penanda atau faktor penyebab perkembangan penyakit. Selain itu, studi komparatif yang lebih luas dengan melibatkan kelompok pasien RA dari berbagai daerah di Indonesia perlu dilakukan untuk memahami variasi komposisi mikrobiota usus dan hubungannya dengan karakteristik klinis penyakit. Terakhir, penelitian intervensi yang melibatkan pemberian probiotik atau prebiotik yang spesifik, dengan tujuan memodulasi komposisi mikrobiota usus dan mengurangi gejala RA, dapat memberikan wawasan baru mengenai potensi terapi berbasis mikrobiota untuk penyakit ini. Penelitian-penelitian ini diharapkan dapat memberikan pemahaman yang lebih mendalam mengenai peran mikrobiota usus dalam patogenesis RA dan membuka jalan bagi pengembangan strategi pencegahan dan pengobatan yang lebih efektif.

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