JURNALFKIPUNTADJURNALFKIPUNTAD

Jurnal Akademika KimiaJurnal Akademika Kimia

Cytotoxic activity is a process that can induce cell death. One of the plants that has cytotoxic properties is bandotan (Ageratum conyzoides L.). The purpose of this study was to identify the class of secondary metabolites present in the ethanol extract of Bandotan herb and to evaluate its cytotoxicity by determining the LC50 value using the Brine Shrimp Lethality Test (BSLT) method. This research includes phytochemical screening and cytotoxicity testing of an ethanol extract of Herba bandotan using the BSLT method. BSLT is the first method for cytotoxicity testing using Artemia salina Leach larvae at the nauplii stage. Used in the BSLT method because it has similar responses with mammals, namely DNA-dependent RNA polymerase in Artemia salina Leach, similar to DNA-dependent RNA polymerase in mammals. By phytochemical screening, the secondary metabolites in Herba Bandotan (Ageratum conyzoides L.) are alkaloids, flavonoids, saponins, steroids, and tannins. Based on the results of the cytotoxicity test using the BSLT method, it shows that the ethanol extract of Herba bandotan (Ageratum conyzoides L.) has the potential to be anti-cancer because it has an LC50 value of 255.1915 g / mL, which is included in the toxic category and has the potential to be anti-cancer. Potential as an anticancer agent in the BSLT test if it has an LC50 value < 1000 µg / mL.

The research identified alkaloids, flavonoids, saponins, steroids, and tannins as secondary metabolite compounds in the bandotan herb.The ethanol extract of bandotan herb exhibited cytotoxicity, indicated by an LC50 value of 255.These findings suggest the potential of bandotan herb as an anti-cancer agent.

Penelitian lebih lanjut perlu dilakukan untuk mengisolasi dan mengidentifikasi senyawa spesifik dalam ekstrak bandotan yang bertanggung jawab atas aktivitas sitotoksiknya, sehingga dapat memahami mekanisme aksi antikanker secara lebih mendalam. Studi lanjutan dapat difokuskan pada pengujian ekstrak bandotan atau senyawa aktifnya pada berbagai jenis sel kanker secara in vitro untuk menentukan efektivitasnya terhadap berbagai jenis kanker. Selain itu, penelitian in vivo menggunakan model hewan diperlukan untuk mengevaluasi keamanan dan efikasi ekstrak bandotan sebagai agen antikanker potensial, termasuk studi tentang dosis optimal dan efek samping yang mungkin terjadi. Penelitian ini diharapkan dapat memberikan dasar ilmiah yang kuat untuk pengembangan obat antikanker baru berbasis bahan alami, khususnya dari tanaman bandotan.

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