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Infectious diseases caused by bacterial and fungal pathogens remain a major public health concern, particularly in the context of increasing antimicrobial resistance. This study aimed to evaluate the in vitro antimicrobial bioactivity of Pseudomonas azotoformans UICC B-91 extract against Salmonella typhi and Microsporum canis. A quantitative experimental design with a completely randomized design (CRD) was applied using three extract concentrations (30%, 50%, and 100%). Antibacterial activity against S. typhi was assessed using the disc diffusion method, while antifungal activity against M. canis was evaluated using the agar well diffusion method. Inhibition zones were measured and analyzed using SPSS with one-way ANOVA. The results showed that the extract exhibited weak antibacterial activity against S. typhi, with inhibition zones ranging from 1.30 ± 0.28 mm to 2.00 ± 0.71 mm, and statistical significance observed only at the 50% concentration (p = 0.039). In contrast, moderate antifungal activity against M. canis was observed, with a maximum inhibition zone of 6.50 ± 0.71 mm, and ANOVA confirmed a significant effect among treatments (F = 803.936; p = 0.026). These findings indicate that Pseudomonas azotoformans UICC B-91 extract contains bioactive compounds with measurable antimicrobial activity under in vitro conditions, particularly against dermatophyte fungi.

The study demonstrated that the Pseudomonas azotoformans UICC B-91 extract exhibited weak antibacterial activity against Salmonella typhi and moderate antifungal activity against Microsporum canis.Statistical analysis confirmed the significant antifungal activity against M.canis, while the antibacterial effect against S.These findings suggest the potential of P.azotoformans as a source of natural antifungal agents, but further research is needed to confirm clinical effectiveness and safety.

Penelitian lanjutan perlu dilakukan untuk mengisolasi dan mengidentifikasi senyawa bioaktif spesifik dalam ekstrak Pseudomonas azotoformans UICC B-91 yang bertanggung jawab atas aktivitas antimikroba, serta menguji efektivitasnya secara individual dan sinergis. Selain itu, studi lebih lanjut diperlukan untuk mengeksplorasi potensi penggunaan ekstrak ini dalam formulasi antimikroba baru, seperti salep atau larutan, untuk pengobatan infeksi jamur kulit yang disebabkan oleh Microsporum canis. Terakhir, penelitian in vivo pada model hewan diperlukan untuk mengevaluasi efikasi, toksisitas, dan profil farmakokinetik ekstrak, serta untuk menentukan apakah ekstrak tersebut dapat mengatasi resistensi antimikroba pada bakteri dan jamur patogen.

  1. Antimicrobial Resistance in Salmonella Typhi Isolated From a Referral Hospital of Kathmandu, Nepal -... journals.sagepub.com/doi/10.1177/11786361211056350Antimicrobial Resistance in Salmonella Typhi Isolated From a Referral Hospital of Kathmandu Nepal journals sagepub doi 10 1177 11786361211056350
  2. Bioactivity of Pseudomonas azotoformans UICC B-91 as an agent with antimicrobial activity, Salmonella... ejurnal.uibu.ac.id/index.php/edubiotik/article/view/1359Bioactivity of Pseudomonas azotoformans UICC B 91 as an agent with antimicrobial activity Salmonella ejurnal uibu ac index php edubiotik article view 1359
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