Publisher

APPERTANIAPPERTANI

Journal Jurnal Ilmu Ternak dan VeterinerJurnal Ilmu Ternak dan Veteriner Abstract

Fowl adenovirus (FAdV) serotype 8b is a pathogenic viral agent of inclusion body hepatitis (IBH) in chickens with high mortality in affected farms. The objective of this study is to determine the pathological changes and to detect FAdV by molecular method in organs of specific-pathogen-free (SPF) chickens infected with FAdV serotype 8b. FAdV isolate, UPM1901, was inoculated into day-old chicks via oral route followed by sampling at 0 hour (h) for control group and subsequently at 12h, 24h, days 3 and 7 post-inoculation (pi). Body weight, liver and bursa of Fabricius weights were measured followed by organs collection for histopathological examination and virus detection using polymerase chain reaction (PCR). It showed that chickens in the infected group were depressed and inappetence prior sudden death with 48% total mortality within day 5pi. Mean body weight was significantly low (p<0.05) than control group at days 3 and 7pi. Swollen, haemorrhages and necrosis of liver were recorded with numerous basophilic intranuclear inclusion bodies in the hepatocytes along with haemorrhages spleen and kidney at 3 dpi onward. FAdV nucleic acid detected in bone marrow at 12h pi followed by the other organs at 24h pi in spleen, liver, and cecal tonsils. At days 3 and 7pi, viral DNA was detected in all the selected organs. This study proves that pathogenic FAdV serotype 8b caused chick`s mortality, pathological changes with existence of viral DNA at 12h pi in lymphoid organ prior distributed to other organs and necessitates effective control strategies against the disease.

Conclusion

This study proves that pathogenic FAdV serotype 8b causes mortality in chicks, induces pathological changes, and exhibits viral DNA presence as early as 12 hours post-infection in lymphoid organs before spreading to other organs.These findings highlight the need for effective control strategies against the disease.The study demonstrates the importance of understanding the viruss impact on lymphoid organs and other tissues for better disease management.Therefore, effective control strategies, such as vaccination programs, are needed to overcome disease and immunosuppressive effects in poultry flocks.

Future Research

Penelitian lebih lanjut perlu dilakukan untuk mengeksplorasi efektivitas berbagai jenis vaksin terhadap FAdV serotipe 8b, termasuk vaksin yang menargetkan respons imun seluler dan humoral. Studi ini dapat membantu mengembangkan strategi vaksinasi yang lebih efektif untuk mencegah penyebaran penyakit. Selain itu, penelitian tentang mekanisme molekuler yang mendasari patogenesis FAdV serotipe 8b, khususnya interaksi virus-inang pada tingkat seluler dan molekuler, sangat penting. Pemahaman yang lebih baik tentang bagaimana virus ini menginfeksi dan merusak sel-sel ayam dapat mengarah pada pengembangan terapi antivirus baru. Terakhir, penelitian epidemiologi yang komprehensif diperlukan untuk memantau penyebaran FAdV serotipe 8b di berbagai wilayah geografis dan mengidentifikasi faktor-faktor risiko yang berkontribusi terhadap wabah penyakit, sehingga dapat dirancang program pengendalian yang lebih tepat sasaran dan efektif.

References

1. Fowl Adenovirus Serotype 4 Induces Hepatic Steatosis via Activation of Liver X Receptor-α | Journal... journals.asm.org/doi/10.1128/JVI.01938-20Fowl Adenovirus Serotype 4 Induces Hepatic Steatosis via Activation of Liver X Receptor Journal journals asm doi 10 1128 JVI 01938 20
2. Genetic diversity of fowl adenovirus serotype 8b isolated from cases of inclusion body hepatitis in commercial... ejournal.undip.ac.id/index.php/jitaa/article/view/39883Genetic diversity of fowl adenovirus serotype 8b isolated from cases of inclusion body hepatitis in commercial ejournal undip ac index php jitaa article view 39883
3. :: JVS :: Journal of Veterinary Science. jvs journal veterinary science vet sci jul e42 english published... vetsci.org/DOIx.php?id=10.4142/jvs.2021.22.e42JVS Journal of Veterinary Science jvs journal veterinary science vet sci jul e42 english published vetsci DOIx php id 10 4142 jvs 2021 22 e42

Metadata

File size	1.14 MB
Pages	11
DMCA	Report