UNIK KEDIRIUNIK KEDIRI

Jurnal Agrinika: Jurnal Agroteknologi dan AgribisnisJurnal Agrinika: Jurnal Agroteknologi dan Agribisnis

The paper reports the antioxidant activity of a non-volatile fraction of lime processing by-products. The activity was measured by spectrophotometry to obtain the 50% inhibition concentration (IC50) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The sample was extracted by the maceration method with n-hexane, ethyl-acetate, and ethyl-ethanol. The IC50 values of 681 ppm, 458 ppm, and 2,775 ppm were n-hexane, ethyl-acetate, and ethyl-ethanol. The experiments concluded that the ethyl-acetate extract provides the best inhibition value to scavenge free-radicals DPPH. The HPLC and mass spectroscopy were measured to find out the content and group of active compounds. The significant compounds consisted of naringin, poncirin, or neoponcirin, which are known as antioxidant substances. The result shows the potential application of the lime by-products, its volatile fraction, and the non-volatile fraction, which is the production residue of lime peel. This work can be applied as an alternative to zero-waste lime production, which may benefit the industry and the environment.

The research concludes that the ethyl-acetate extract is the most active antioxidant extract, exhibiting an IC50 of 457.This value is superior to those obtained with n-hexane and ethanol extracts, making ethyl-acetate the preferable solvent for extracting *Citrus aurantifolia* peels.The main antioxidant compounds identified in the extract are poncirin or neoponcirin and naringin, confirming the potential of lime peel as an antioxidant product.

Penelitian lebih lanjut dapat dilakukan untuk menguji efektivitas ekstrak kulit limau sebagai aditif alami dalam produk makanan untuk meningkatkan stabilitas oksidatif dan memperpanjang umur simpan. Selain itu, studi komparatif perlu dilakukan untuk membandingkan metode ekstraksi yang berbeda, seperti ekstraksi berbantuan ultrasonik atau ekstraksi fluida superkritis, untuk mengoptimalkan perolehan senyawa antioksidan dan efisiensi proses. Terakhir, penelitian mendalam mengenai mekanisme aksi senyawa antioksidan spesifik yang terkandung dalam ekstrak kulit limau, seperti naringin dan poncirin, pada tingkat seluler dan molekuler akan memberikan pemahaman yang lebih baik tentang potensi manfaat kesehatan dan membuka jalan bagi pengembangan aplikasi farmasi atau nutraseutikal yang inovatif.

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