INSTITUTSUNANDOEINSTITUTSUNANDOE

Journal of Multidisciplinary ScienceJournal of Multidisciplinary Science

Compost is one of the potential microbial habitats for the discovery of a wide range of novel biocatalysts. Compost samples, originally made of plant litter, were collected at the Expo Park (Osaka, Japan). The temperature of the composts is around 50 °C. Metagenomic DNA was isolated from compost and was used to amplify partial 16S rRNA genes (rDNAs) by polymerase chain reaction for investigation of the microbial diversity in the compost. Molecular microbial identification was carried out by metagenome analysis of 16S rRNA using MinION Next Generation Sequencing (NGS). The results indicated that branch-leaf compost sample from Expo Park is dominated by thermophilic microorganisms. The majority of bacteria are Caldicoprobacter, Hungateiclostridium, and Thermoclostridium. In conclusion, thermophilic microorganisms are not only found in extreme environments; they are also present even in moderate temperatures.

The analysis of 16S sequencing demonstrated that the branch-leaf compost sample from Expo Park is predominantly inhabited by thermophilic microorganisms.Specifically, the bacterial community is largely composed of Caldicoprobacter, Hungateiclostridium, and Thermoclostridium.These findings suggest that thermophilic microorganisms can thrive not only in extreme environments but also in conditions with moderate temperatures.

Penelitian lanjutan dapat dilakukan untuk mengidentifikasi dan mengkarakterisasi secara lebih rinci potensi enzim yang dihasilkan oleh mikroorganisme termofilik yang dominan dalam kompos daun dan cabang ini, misalnya enzim selulase atau ligninase untuk degradasi biomassa. Selain itu, studi yang lebih mendalam diperlukan untuk memahami interaksi antar mikroorganisme dalam komunitas kompos dan bagaimana interaksi tersebut berkontribusi terhadap proses dekomposisi dan pembentukan humus. Selanjutnya, penelitian dapat fokus pada optimasi kondisi lingkungan (seperti suhu, kelembaban, dan rasio C/N) untuk meningkatkan pertumbuhan dan aktivitas mikroorganisme termofilik yang bermanfaat dalam proses pengomposan, sehingga menghasilkan kompos dengan kualitas yang lebih tinggi dan efisiensi yang lebih baik. Penelitian ini akan memperluas pemahaman mengenai ekologi mikroba dalam kompos dan berpotensi menghasilkan aplikasi bioteknologi yang inovatif dalam pengelolaan limbah organik dan produksi pupuk hayati.

  1. Frontiers | Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics. frontiers gut microbiome... doi.org/10.3389/fmicb.2016.00459Frontiers Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics frontiers gut microbiome doi 10 3389 fmicb 2016 00459
Read online
File size448.44 KB
Pages8
DMCAReport

Related /

ads-block-test