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Majalah Obat TradisionalMajalah Obat Tradisional

Virgin olive oil contains phenolic compounds that were potential for anti-inflammatory and cancer treatment. Computational biology is a beneficial method to understand how this compound can affect the biological process in humans. This research is conducted by the potential screening of VOO compounds, constructing the pharmacological network and enrichment, and docking simulation. The enrichment result showed that the EGFR, BRAF, MAPK1, CCND1, and MDM2 protein have multiple cancer contributions and related pathways. The docking simulation result showed that the interaction of EGFR-luteolin, BRAF-luteolin, MAPK1-luteolin, CCND1-apigenin, and MDM2-1-hydroxypinoresinol has the highest binding affinity. Further research with the in-vitro methods are required to check the reliable mechanisms of each compound to their protein target.

The VOO compounds have multiple protein targets on various pathways, especially in cancers and tumors.Five proteins that act on multiple and central pathways from KEGG and WikiPathways database were MAPK1, BRAF, EGFR, MDM2, and CCND1.The docking simulation showed that the luteolin compound was stable with EGFR protein and BRAF protein, with the docking score reaching about -9.1-hydroxypinoresinol was stable to interact with MDM2 protein, reaching a score of -7.apigenin and stearic acid were stable to interact with CCND1 protein with docking score reaching -6.Also, MAPK protein is stably interacting with luteolin, which has a docking score of -9.Those compounds, especially luteolin, probably have the potential for therapeutic on various cancers and tumors.These docking simulation results also report that luteolin has a stronger binding affinity than the control compound for interfering with the BRAF and MAPK protein.The in-vitro and in-vivo study must be carried out to validate the specific response from ligands on proteins involved in cancer pathways.

Berdasarkan penelitian ini, beberapa saran penelitian lanjutan yang dapat dikembangkan antara lain adalah melakukan studi in vitro untuk memvalidasi mekanisme kerja senyawa-senyawa dari minyak zaitun perawan terhadap target protein yang telah diidentifikasi. Selain itu, penelitian lebih lanjut dapat difokuskan pada identifikasi senyawa-senyawa spesifik dalam minyak zaitun perawan yang paling efektif dalam menghambat pertumbuhan sel kanker, serta mengeksplorasi potensi kombinasi senyawa-senyawa tersebut dengan terapi kanker konvensional untuk meningkatkan efektivitas pengobatan. Terakhir, penelitian juga dapat dilakukan untuk menginvestigasi efek senyawa-senyawa dari minyak zaitun perawan terhadap pembentukan metastasis kanker, dengan tujuan untuk mengembangkan strategi pencegahan dan pengobatan metastasis yang lebih efektif.

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