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Morel berry root contains flavonoids, tannins, and alkaloids that have antibacterial characteristics. This study aims to determine the antibacterial activity of the morel berry root ethanol fraction against Staphylococcus epidermidis and Pseudomonas aeruginosa and the most effective active compounds to inhibit such activity. Extractions of morel berry root used the maceration method with ethanol 70% and then continued by fractionation using ethanol, chloroform, and n-hexane. Test of the compound fraction was performed using Thin Layer Chromatography (TLC) and characterization with FTIR Spectrophotometer. The results of the antibacterial activity with inhibitory zone diameter were at a 100% concentration of 18.69 mm in S. epidermidis while P. aeruginosa of 20.00 mm. Bioautographic results for ethanol fraction indicate an inhibited zone with an Rf value of 0.72 in the S. epidermidis and P. aeruginosa which is thought to be a flavonoid compound. Characterization with FTIR spectrophotometer functional groups O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic in ethanol fraction with Rf 0.72.

The ethanol fraction of morel berry root exhibits antibacterial activity against both Staphylococcus epidermidis and Pseudomonas aeruginosa.The study identified flavonoids as the most effective antibacterial compounds within the ethanol fraction, specifically the flavonone group with an Rf value of 0.FTIR analysis confirmed the presence of functional groups characteristic of flavonoids, including O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic.

Penelitian lebih lanjut perlu dilakukan untuk memurnikan senyawa aktif dari fraksi etanol akar ceplukan menggunakan metode kromatografi kolom, guna mendapatkan senyawa flavonoid yang lebih murni dan terkarakterisasi dengan baik. Selain itu, studi komparatif perlu dilakukan untuk menguji efektivitas fraksi etanol ceplukan terhadap berbagai jenis bakteri patogen lainnya, termasuk bakteri yang resisten terhadap antibiotik konvensional, untuk memperluas potensi aplikasi antibakterinya. Terakhir, pengembangan formulasi sediaan farmasi berbasis fraksi etanol ceplukan, seperti salep atau gel, dapat dilakukan untuk meningkatkan stabilitas dan efikasi senyawa aktif, serta mempermudah aplikasinya sebagai alternatif pengobatan infeksi bakteri pada kulit atau luka.

  1. Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus... journal.ugm.ac.id/TradMedJ/article/view/78171Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root Physalis angulata L Against Staphylococcus journal ugm ac TradMedJ article view 78171
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