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Indonesian Journal of Applied Research (IJAR)Indonesian Journal of Applied Research (IJAR)

Gedong Gincu Mango peel (Mangifera indica L.) contains a variety of secondary metabolites, including flavonoids, tannins, saponins, alkaloids, and terpenoids. These compounds play a crucial role in self-defence and exhibit significant pharmacological activity, particularly as antibacterial agents. The purpose of this study is to identify the content of secondary metabolite compounds of each fraction based on its polarity properties, and to determine the inhibitory power of Staphylococcus aureus ATCC 25293 bacteria. This study is experimental research with a post-test only control group. This study used 2 control groups and 12 treatment groups consisting of n-hexane, ethyl acetate, and butanol fractions with concentrations of 6.25%, 12.5%, 25%, 50% respectively and repeated 3 times. The antibacterial test was carried out by the well diffusion method, which was repeated 3 times on the MHA medium, and the inhibition zone was measured. The study showed that the n-hexane fraction contained alkaloids, steroids, and saponins, while the ethyl acetate and butanol fractions contained tannins, flavonoids, triterpenoids, and phenolics. A 50% concentration produced the greatest inhibition zone in all fractions, with mean values of 8.05 mm (moderate) for n-hexane, 15.41 mm (strong) for ethyl acetate, and 12.16 mm (strong) for butanol. The 50% ethyl acetate fraction was the most effective in inhibiting the growth of Staphylococcus aureus ATCC 25923.

The study concludes that Gedong Gincu Mango Peel contains secondary metabolites with antibacterial properties.The antibacterial activity of each fraction varies according to its polarity, with the ethyl acetate fraction at a concentration of 50% being the most effective in suppressing the growth of Staphylococcus aureus ATCC 25923.These findings suggest the potential of mango peel as a source of antibacterial agents.

Penelitian lebih lanjut perlu dilakukan untuk mengidentifikasi dan mengisolasi senyawa-senyawa spesifik dalam fraksi etil asetat yang bertanggung jawab atas aktivitas antibakteri yang kuat terhadap Staphylococcus aureus. Studi ini dapat melibatkan teknik kromatografi lanjutan dan analisis spektroskopi untuk menentukan struktur kimia senyawa-senyawa tersebut. Selain itu, penelitian in vivo pada model hewan diperlukan untuk mengevaluasi efikasi dan keamanan fraksi etil asetat sebagai agen antibakteri potensial. Penelitian selanjutnya juga dapat mengeksplorasi potensi kombinasi fraksi etil asetat dengan antibiotik konvensional untuk mengatasi masalah resistensi antibiotik, serta menguji efektivitasnya terhadap strain Staphylococcus aureus yang resisten terhadap berbagai antibiotik. Terakhir, studi formulasi dapat dilakukan untuk mengembangkan sediaan topikal atau oral yang stabil dan efektif berdasarkan fraksi etil asetat untuk aplikasi klinis.

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