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Jurnal Ilmu Ternak dan VeterinerJurnal Ilmu Ternak dan Veteriner

The integrity of the sperm plasma membrane in frozen semen is crucial in assessing semen quality and is directly linked to fertility. The plasma membrane integrity of sperm can be tested using several methods, including the Hypo-Osmotic Swelling Test (HOS-Test) and Carboxyfluorescein Diacetate-Propidium Iodide Staining (CFDA-PI). This study compares the integrity of the plasma membrane of frozen Murrah buffalo semen using the HOS-Test and CFDA-PI. The semen was obtained from four buffalo bulls, each representing a different production batch. The straw was thawed individually, and quality was assessed. The plasma membrane integrity of each sample was evaluated using the HOS-Test and CFDA-PI staining methods. A two-sample t-test was used to analyze the data and demonstrate that there is a statistically significant difference in sperm plasma membrane integrity between the HOS-Test (64.83%) and CFDA-PI staining (58.97%) (P<0.05). The HOS-Test assessment is optimal for cost-effectiveness, speed, and convenience. It can be implemented in laboratory and field settings with equal ease. In contrast, CFDA-PI staining is a good standard for accuracy and precision. It provides a comprehensive foundation for determining the quality of frozen semen.

This studys findings indicate notable differences between the HOS-Test and CFDA assessments of frozen Murrah buffalo semen.Both methods are appropriate for detecting plasma membrane integrity, but the selection of the most suitable method can be adjusted according to specific requirements.The HOS-Test is a rapid, cost-effective, and practical method for use in laboratory and field settings.

Further research could investigate the correlation between sperm plasma membrane integrity, as assessed by both HOS-Test and CFDA-PI, and *in vitro* fertilization rates in Murrah buffaloes to determine which method best predicts fertilization success. Additionally, studies could explore the impact of different cryopreservation protocols on sperm plasma membrane integrity, aiming to optimize semen freezing techniques for improved post-thaw quality. Finally, research could focus on identifying specific biomarkers within the sperm plasma membrane that correlate with membrane integrity and fertility potential, potentially leading to the development of more sensitive and accurate diagnostic tools for assessing semen quality in buffalo breeding programs.

  1. Cryodamage of plasma membrane and acrosome region in chicken sperm - Olexikova - 2019 - Anatomia, Histologia,... doi.org/10.1111/ahe.12408Cryodamage of plasma membrane and acrosome region in chicken sperm Olexikova 2019 Anatomia Histologia doi 10 1111 ahe 12408
  2. Brazil - Development of assays for the characterization of sperm motility parameters, viability, and... doi.org/10.1590/1984-3143-AR2023-0113Brazil Development of assays for the characterization of sperm motility parameters viability and doi 10 1590 1984 3143 AR2023 0113
  3. "Storage temperature of boar semen and its relationship to changes in s" by DARIUSZ GACZARZEWICZ,... journals.tubitak.gov.tr/biology/vol39/iss4/9Storage temperature of boar semen and its relationship to changes in s by DARIUSZ GACZARZEWICZ journals tubitak gov tr biology vol39 iss4 9
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