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JOURNAL JPSJOURNAL JPS

Journal Journal of Pharmaceutical and SciencesJournal of Pharmaceutical and Sciences Abstract

Background: Erythrina subumbrans (Hassk.) Merr, locally known as dadap serep, is a medicinal plant traditionally used to treat various ailments, indicating the presence of bioactive compounds. However, scientific exploration of its phytochemical profile and antioxidant potential remains limited compared to other species in the genus. Objective: This study aimed to (1) identify the bioactive compound groups in the 70% ethanol extract of Erythrina subumbrans leaves through phytochemical screening and Thin Layer Chromatography (TLC), and (2) evaluate its antioxidant activity quantitatively using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Methods: The leaves were extracted via maceration using 70% ethanol. The extract was subjected to standard phytochemical screening tests for flavonoids, alkaloids, saponins, and tannins. Flavonoid profiling was performed using TLC with a quercetin standard. Antioxidant activity was assessed by the DPPH method, with vitamin C as a reference standard, and the IC₅₀ value was determined. Results: Phytochemical screening confirmed the presence of flavonoids, alkaloids, saponins, and tannins. TLC analysis indicated the presence of flavonoid compounds, with one major spot showing an Rf value similar to the quercetin standard. The DPPH assay revealed that the extract possesses strong antioxidant activity, with an IC₅₀ value of 49.94 ppm. According to established antioxidant strength criteria, this IC₅₀ value is categorized as very strong. Conclusion: The 70% ethanol extract of Erythrina subumbrans leaves contains diverse bioactive compounds, particularly flavonoids, and exhibits very strong antioxidant activity. These findings scientifically support its traditional use and highlight its potential as a promising source of natural antioxidants for further pharmaceutical or nutraceutical development.

Conclusion

The 70% ethanol extract of Erythrina subumbrans (Hassk.) Merr leaves contains various classes of secondary metabolites, including flavonoids, alkaloids, saponins, and tannins.Thin-layer chromatography (TLC) analysis provided preliminary evidence of flavonoid compounds, with one major constituent displaying chromatographic behavior similar to the quercetin standard.The extract demonstrated significant radical scavenging activity against DPPH, with an IC₅₀ value of 49.94 µg/mL, categorizing it as possessing very strong antioxidant capacity.

Future Research

Further research should focus on isolating and identifying the specific flavonoid compounds responsible for the observed antioxidant activity using advanced analytical techniques like HPLC or LC-MS/MS. Investigating the potential synergistic effects between different bioactive compounds present in the extract, such as flavonoids, alkaloids, and saponins, on antioxidant activity and other pharmacological properties is crucial. Additionally, studies exploring the in vivo efficacy of the extract in relevant disease models, such as oxidative stress-induced conditions, are warranted to validate its therapeutic potential and assess its bioavailability and metabolic fate within the body. These investigations will contribute to a more comprehensive understanding of Erythrina subumbranss medicinal properties and pave the way for its development as a novel natural antioxidant source for pharmaceutical and nutraceutical applications, potentially leading to innovative strategies for preventing and treating oxidative stress-related diseases.

References

1. Journal of Pharmaceutical and Sciences. journal sciences quick jump page content main navigation sidebar... doi.org/10.36490/journal-jps.comJournal of Pharmaceutical and Sciences journal sciences quick jump page content main navigation sidebar doi 10 36490 journal jps
2. Identification of Active Compounds in Dadap Serep Leaves (Erythrina subumbrans (Hassk.) Merr) and Their... doi.org/10.36490/journal-jps.com.v9i1.1164Identification of Active Compounds in Dadap Serep Leaves Erythrina subumbrans Hassk Merr and Their doi 10 36490 journal jps v9i1 1164

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