Publisher

STIFARSTIFAR

Journal Media Farmasi IndonesiaMedia Farmasi Indonesia Abstract

Diabetes mellitus is a chronic metabolic disorder caused by high blood glucose levels. One common complication in diabetes mellitus sufferers is diabetic ulcers. Research has shown that pineapple skin contains flavonoid, an active compound with anti‑inflammatory and antimicrobial properties, which can accelerate wound healing. This study aims to determine the effect of applying a hydrogel patch containing pineapple peel extract (Ananas comosus L.) as a wound dressing on male white mice (Mus musculus) with diabetic ulcers. The study involved several stages, including pineapple peel extraction, hydrogel patch preparation, antibacterial activity testing, and application of the hydrogel patch on test animals. The research used 20 male white mice of the DDY strain divided into 5 groups. The negative control group received a hydrogel patch without extract, while the positive control group received Hydrocolloid Dressing. Treatment groups I, II, and III were given hydrogel patches with 10%, 20%, and 30% pineapple peel extract, respectively. The results showed that the hydrogel patch with pineapple peel extract accelerated wound healing in diabetic ulcer models. The antibacterial activity test indicated antimicrobial activity against Staphylococcus aureus, commonly found in diabetic ulcers. The hydrogel patch application reduced inflammation and improved skin tissue structure in test animals, demonstrating the potential of pineapple peel extract hydrogel patches as an effective alternative wound dressing for diabetic ulcers.

Conclusion

Pineapple peel extract is rich in tannins, saponins, flavonoids, terpenoids, phenolics and alkaloids.The antibacterial activity of pineapple peel extract at concentrations of 10%, 20% and 30% against Staphylococcus aureus is weak, with inhibition zones of 0.85 mm respectively, but 20% concentration is superior.In the hydrogel patch test, the formula without extract (control) and the formula with 10% extract were more stable than the other formulas.In the effectiveness test of pineapple peel extract hydrogel patches on test animals, the group with 20% extract achieved the fastest percentage of diabetic ulcer closure compared to other treatment groups.Descriptive tests showed a trend of decreasing wound diameter in test animals given pineapple peel extract hydrogel patches.It can be concluded that pineapple peel extract contains secondary metabolites that have the potential for wound closure.Giving hydrogel patches containing pineapple peel extract at 20% effectively accelerates diabetic ulcer wound closure in test animals.

Future Research

Selanjutnya, penelitian dapat memperluas uji klinis pada manusia dengan mengkaji keamanan dan tolerabilitas patch hidrogel ekstrak kulit nanas pada pasien luka ulkus diabetik, sehingga dapat menilai efek klinisnya secara real‑time. Selain itu, studi komparatif antara patch hidrogel ekstrak nanas dan pengobatan standar (misalnya dressing hidroklorida) dalam skala besar dapat memberi gambaran efektivitas ekonomi dan klinisnya, sehingga dapat memandu kebijakan pengobatan luka diabetes di fasilitas kesehatan. Akhirnya, penelitian dapat mengeksplorasi mekanisme molekuler secara mendalam, seperti pengaruh ekstrak nanas terhadap ekspresi gen‑tulang dan sitokin pro‑ dan anti‑inflamasi, guna memahami jalur penyembuhan luka yang dipicu oleh komponen bioaktif ini, sehingga dapat memperkuat pijakan ilmiahnya.

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Pages	11
DMCA	Report